To investigate the effect of temperature changes to the activity of Enzyme Catalyses.

Coursework: To investigate the effect of temperature changes to the activity of Enzyme Catalyses. By Marcus Final update: 26/11/03 Apparatus needed: Boiling pipeworks Beakers tip with a delivery tube Thermometer 5ml spray (To receive potato) 10ml syringe (To mea convinced(predicate) the amount of solution) D frapped potato Bunsen burner (can be replaced by thermosactic pee lav if possible) Ice Glass rod total heat peroxide pH 7 original Variable and perpetual factors: ØpH of enzyme: This can be make by the humanitarian of pH7 buffer. ØConcentration of enzyme: This can be through by exploitation atomic number 1 peroxide from the alike arguing every time. ØThe time for reaction must be the resembling all(prenominal) time ØSince the effect of changes in temperature to the enzyme activity is investigated in this sweat egress, all factors, except the temperature, has to be constant. I choose to bear the experiment several times with a different temperature of the substrate and the enzyme each time. The temperature chosen be 10?,20?, 30?,40?,50?,60?. The subroutine: 1.First or all, a boil tube is used to collect 5ml of diced potato with the 5ml syringe. 2.Add 10ml of pH7 buffer to the boiling tube to make sure pH is constant. 3.Pour 10ml of hydrogen peroxide into another boiling tube. 4.

prop both boiling tube in a pee bath, add heat to the water bath until it reaches 80 ?. (For 10?. And 20?., which is probably get down than room temperature, ice is added to the water bath instead of heating.). Or If thermostatic water bath is available, the temperature can be changed by panorama th e thermostatic water bath. When the temperat! ure is reached, take both boiling tubes out of the thermostatic water bath and continue the experiment. 5.Take both boiling tube out of the water bath, make sure that their temperature are the same. 6.Get unsex with... If you want to get a full essay, articulate it on our website: BestEssayCheap.com

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